The soil bacterium Pseudomonas putida KT2440 is a "generally recognized as safe"-certified strain with robust property and versatile metabolism. Thus， it is an ideal candidate for synthetic biology， biodegradation， and other biotechnology applications. The known genome editing approaches of Pseudomonas are suboptimal; thus， it is necessary to develop a high efficiency genome editing tool.
The soil bacterium Pseudomonas putida KT2440 is a "generally recognized as safe"-certified strain with robust property and versatile metabolism. Thus， it is an ideal candidate for synthetic biology， biodegradation， and other biotechnology applications. The known genome editing approaches of Pseudomonas are suboptimal; thus， it is necessary to develop a high efficiency genome editing tool.
